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Anti-TTF1 - Rabbit (WRAB-1231)使用說(shuō)明
產(chǎn)品信息
品牌 | Seven Hills Bioreagents |
貨號(hào) | WRAB-1231 |
規(guī)格 | 100 µL |
宿主 | 兔 |
反應(yīng)種屬 | 人、鼠 |
克隆類(lèi)型 | 多克隆 |
偶聯(lián)物 | 無(wú) |
緩沖液 | 0.01% sodium azide |
抗原 | 重組鼠TTF-1融合6*his蛋白 |
保存條件 | 儲(chǔ)存在-20℃，避免反復(fù)凍融 |
蛋白背景 | 甲狀腺轉(zhuǎn)錄因子-1（TTF-1）是同源域轉(zhuǎn)錄因子家族的成員，并且在調(diào)節(jié)甲狀腺，肺和腦內(nèi)表達(dá)的基因中起作用。 |
產(chǎn)品使用
實(shí)驗(yàn)應(yīng)用 | 建議稀釋倍數(shù) |
Western Blot | 1: 5000 |
Immunohistochemistry | 1:1000 - 1:2000 |
說(shuō)明：最佳稀釋倍數(shù)與濃度應(yīng)由研究人員確認(rèn)
應(yīng)用實(shí)例
圖片為成年小鼠肺組織，用6%正常山羊血清預(yù)包埋，并用抗大鼠TTF-1兔血清（1:2000稀釋?zhuān)┤旧Ｍㄟ^(guò)DAB（黑色）觀察TTF-1，并用核快紅（粉紅色）復(fù)染。放大倍數(shù)10倍。
參考文獻(xiàn)
1. Zhou L, Lim L, Costa RH, Whitsett JA. 'Thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein B, C, and Clara cell secretory protein in developing mouse lung.' J Histochem Cytochem. 1996 Oct;44(10):1183-93.
2. Stahlman MT, Gray ME, Whitsett JA. 'Expression of thyroid transcription factor-1(TTF-1) in fetal and neonatal human lung.' J Histochem Cytochem. 1996 Jul;44(7):673-8.
3. Khoor A, Whitsett JA, Stahlman MT, Olson SJ, Cagle PT. 'Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma.' Hum Pathol. 1999 Jun;30(6):695-700.
4. deFelice M, Silberschmidt D, DiLauro R, Xu Y, Wert SE, Weaver TE, Bachurski CJ, Clark JC, Whitsett JA. 'TTF-1 phosphorylation is required for peripheral lung morphogenesis, perinatal survival, and tissue-specific gene expression.' J Biol Chem. 2003 Sep 12;278(37):35574-83.
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